Equipment to understand the effect of calcium-sensing receptor activation

Calcium is maintained within a tight physiological range by calcium sensing receptor. The exact functions of the receptor in kidney have not been determined in sufficient detail and we have new indications about how they are activated. We received a generous grant from the Carlsberg Foundation for an ion chromatography system that allows measurements of all relevant anions and cations, whereas we have previously determined all divalent cations and anions by colorimetric assays. The system works with an autosampler, so that much less hands-on activity is required. In this project we aim to acquire an additional ion chromatography column, as this will expand system capabilities even more, to allow simultaneous measurements of both types of ions from the same sample preparation.